Mouse Anti-Human CD61

100ug (NP20077) Please inquiry

Antibody type:Flowcytometry

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:FCM

Purify method:

Species:Human

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Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:7F7-D9

Dilution:

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Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100μl EDTA抗凝的人外周血加入流式测定管底部;2. 加入适量纯化抗体与全血混匀，室温孵育30分钟;3. 加入2 ml 1×红细胞裂解液混匀后避光置10分钟,溶解红细胞;4. 将试管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;6. 加入适量荧光标记抗小鼠免疫球蛋白试剂，室温避光孵育20分钟;7. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;8. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:7F7-D9单抗识别人CD 61，也被称为整合素β3和GPIIIa。CD 61大小为 90 - 110KD隶属整合素 β家庭。广泛表达于多种细胞，包括白细胞，血小板，内皮细胞和平滑肌细胞。CD 61与α整合素CD 41和CD51非共价键结合，形成αIIbβ3（CD 41 / CD 61）和αvβ3（CD51 / CD 61）复合物。α和β异二聚体能够调解不同的细胞反应，包括粘附，增殖和分化。CD41 / CD 61整合素已被证明参与血小板聚集，并结合纤维蛋白原，血管性血友病因子和纤维连接蛋白。CD51 / CD 61整合素结合基质蛋白包括粘连蛋白，纤维连接蛋白，血管性血友病因子和纤维蛋白原，由此可见，它在调节迁移和血管内皮细胞生存方面具有重要的作用。

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.