Mouse Anti-Human CD69
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Antibody type:Flowcytometry
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:FCM
Purify method:
Species:Human
Gene Name:
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Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:3F2-F6
Dilution:
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Sequence Similarity:
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Storage:短期内经常使用时,请将抗体2-8℃保存,至少可以保存6个月。若长期保存,请将抗体分装后-20℃至-80℃保存,切忌反复冻融。
Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3
Background:1. 取100μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液(Cat.:FXP005)重悬细胞,1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3;Cat.:FXP005][细胞固定液:2%甲醛溶液]
Molar Function:3F2-F6 单克隆抗体特异性识别人细胞表面CD69,也被称为早期活化抗原(VEA)。CD69大约30 kDa 和作为一个二硫化物连接的二聚体在细胞表面表达。CD69 迅速上调激活作用并在淋巴细胞,单核细胞和血小板上表达
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.