Mouse Anti-Human CD7 (FITC)

100ug (NP20149) Please inquiry

Antibody type:Flowcytometry

Label:FITC

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:FCM,IF

Purify method:

Species:Human

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Clonality:Monclonal antibody

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Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5H7-C8

Dilution:

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Storage:短期内经常使用时，请将抗体2-8℃保存，至少可以保存6个月。若长期保存，请将抗体分装后-20℃至-80℃保存，切忌反复冻融。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 取100 μl EDTA抗凝的人外周血加入5 ml流式管底;2. 取10 μl标记抗体加入流式管底与全血混匀,室温避光孵育20分钟;3. 加入2ml 1×红细胞裂解液混匀后避光放置10分钟,溶解红细胞;4. 将样品管置离心机中以1000 rpm离心5分钟后弃上清;5. 加入2 ml PBS洗液重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品应在当天上机测定,也可加固定液4℃保存过夜测定).[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]

Molar Function:单抗2C12-B5单抗能够特异性识别人CD7分子，也被称为gp40、 Leu9，分子量为40 kD，是免疫球蛋白基因家族的成员。其N-端氨基酸序列（aa：107）与Ig κ链高度同源。C-端氨基酸是在膜外部分，富含脯氨酸。CD7在多数未成熟和成熟T淋巴细胞、少数急性髓系白血病均有表达，还发现在NK细胞,小部分B细胞亚群、恶性B细胞上有表达。据报道，人类CD34+CD38-脐带血细胞表达CD45RA、HLA-DR和高水平的CD7。这些细胞具有淋巴细胞增殖的能力。可以产生B细胞、,NK细胞和DC细胞,但不具备发展成髓细胞或红色的细胞的能力。CD7+表型区分原始人类淋巴祖细胞的多能干细胞。此外，有人发现结合CD7和 CD28 来分析人Treg细胞，当老鼠缺乏CD28和CD7时，Treg总数有所减少，同时也抑制了Treg的活性。

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.