Mouse Anti-Human IFN-γ (PE)

Antibody type:Flowcytometry

Label:PE

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:FCM

Purify method:

Species:Human

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:2-8℃避光保存，勿冰冻。

Buffer condition:0.01MPBS,pH7.4,0.2%(w/v)BSA,0.09%(w/v)NaN3

Background:1. 提取人单个核细胞（PBMCs），用RPMI-1640调整细胞数为1×107/ml;2. 加入细胞刺激剂，37℃、5％CO2孵箱内培养4-6小时;3. 收集细胞，用PBS洗一次;4. 用固定液、破膜剂处理细胞后，加入10ul IFN-γ, PE标记抗体，室温避光反应20分钟;5. 加入2 ml 破膜剂重悬细胞，1000 rpm离心5分钟,弃上清;6. 加入0.5 ml PBS洗液重悬细胞, 上流式细胞仪检测。

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.