Mouse CAMK4 / CaMKIV Protein (His & GST Tag)

A430110E23Rik,AI666733,CaMKIV,CaMKIV/Gr,D18Bwg0362e

100ug (NPP2665) Please inquiry

Catalog Number	P50838-M20B
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	A430110E23Rik,AI666733,CaMKIV,CaMKIV/Gr,D18Bwg0362e
Molecular Weight	The secreted recombinant mouse CAMK4/GST chimera consists of 706 amino acids and has a calculated molecular mass of 80.4 kDa. The recombinant protein migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 94 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse CAMK4 (P08414) (Met1-Tyr469) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	No Kinase Activity
Research Area	Immunology \|Signal Transduction \|Protein Kinase \|Intracellular Kinase \|Ca2+/Calmodulin-Dependent Protein Kinase (CaM Kinase)
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Ca²⁺/ calmodulin-dependent protein kinase 4 (CAMKⅣ) belongs to the serine/threonine protein kinase family, and to the Ca²⁺/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca²⁺/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Ca²⁺ and then apt to combine with the target protein. Ca²⁺/ calmodulin-dependent protein kinase 4 (CAMKⅣ) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKⅢ and CaMKⅣ have EF-hand structure.
Reference	Feliciano DM, et al. (2009) Repression of Ca²⁺/calmodulin-dependent protein kinase IV signaling accelerates retinoic acid-induced differentiation of human neuroblastoma cells. J Biol Chem. 284 (39): 26466-81. Zhao X, et al. (2001). The modular nature of histone deacetylase HDAC4 confers phosphorylation-dependent intracellular trafficking. J Biol Chem. 276 (37): 35042-8. Racioppi L, et al. (2008) Calcium/calmodulin-dependent kinase IV in immune and inflammatory responses: novel routes for an ancient traveller.