Mouse CD112 / Nectin-2 / PVRL2 Protein (His Tag)
AI325026,AI987993,Cd112,MPH,nectin-2,Pvr,Pvs
- 100ug (NPP1111) Please inquiry
| Catalog Number | P50318-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | AI325026,AI987993,Cd112,MPH,nectin-2,Pvr,Pvs |
| Molecular Weight | The secreted recombinant mouse PVRL2 comprises 331 amino acids with a predicted molecular mass of 36 kDa. As a result of glycosylation, it migrates as an approximately 40-45 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Gln 32 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. 1. Immobilized mouse CD112-His (P 50318-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated mouse DNAM1-His (P 50232-M08H) with a linear range of 0.156-5.0 μg/ml. 2. Immobilized mouse CD112-His (P 50318-M08H) at 10 μg/ml (100 μl/well) can bind mouse DNAM1-Fc (P 50232-M03H) with a linear range of 0.03-1.0 μg/ml. |
| Research Area | Developmental Biology |Embryogenesis |Germ Layer Formation |Ectoderm Marker |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy. |
| Reference |