Mouse CD137 / 4-1BB / TNFRSF9 Protein (Fc Tag)
4-1BB,A930040I11Rik,AA408498,AI325004,Cd137,CDw137,ILA,Ly63
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| Catalog Number | P50811-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | 4-1BB,A930040I11Rik,AA408498,AI325004,Cd137,CDw137,ILA,Ly63 |
| Molecular Weight | The secreted recombinant mouse TNFRSF9/Fc is a disulfide-linked homodimer. The reduced monomer comprises 429 amino acids and has a calculated molecular mass of 47 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Val 24 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse TNFRSF9 (NP_001070976.1) (Met 1-Leu 211) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized mouse His-TNFSF9 (P50067-M07H) at 10 μg/ml (100 μl/well) can bind mouse TNFRSF9-Fc, The EC50 of mouse TNFRSF9-Fc is 12.0-29.0 ng/ml. |
| Research Area | Cardiovascular |Angiogenesis |Cytokine & Receptor |Tumor Necrosis Factor & Receptor |TNF receptor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. |
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