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Mouse CD16-2 / FCGR4 Protein (His & AVI Tag)

4833442P21Rik,CD16-2,FcgammaRIV,Fcgr3a,FcgRIV,Fcrl3

Catalog Number P50036-M27H
Organism Species Mouse
Host Human Cells
Synonyms 4833442P21Rik,CD16-2,FcgammaRIV,Fcgr3a,FcgRIV,Fcrl3
Molecular Weight The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.
predicted N Glu 19
SDS-PAGE
Purity > 97 % as determined by SDS-PAGE
Protein Construction The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Bio-activity Measured by its binding ability in a functional ELISA.
Immobilized mouse CD16-2-AVI-His (cat:50036-M27H) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (P10702-HNAC). The EC50 of human IgG1 (Fc) (P10702-HNAC) is 0.34-0.78 μg/ml.
Research Area Immunology |Cluster of Differentiation (CD) |Natural Killer (NK) Cell CD Antigen
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference