Mouse CD16 / FCGR3 Protein (His Tag)
CD16
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Catalog Number | P50326-M08H |
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Organism Species | Mouse |
Host | Human Cells |
Synonyms | CD16 |
Molecular Weight | The secreted recombinant mouse FCGR3 consists of 196 amino acids and has a predicted molecular mass of 22.6 kDa. As a result of glycosylation, the apparent molecular mass of rmFCGR3 is approximately 35-40 kDa in SDS-PAGE under reducing conditions. |
predicted N | Ala 31 |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the mouse FCGR3 (P08508-1) extracellular domain (Met 1-Thr 215) was fused with a polyhistidine tag at the C-terminus. |
Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR3-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.12-0.28 μg/ml. |
Research Area | Signaling |Signal Transduction |ITIM/ITAM Immunoreceptors and Related Molecules |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
Reference |