Mouse CD28 Protein (His & Fc Tag)
Cd28, CH29-189K17.3
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Catalog Number | P50103-M03H |
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Organism Species | Mouse |
Host | Human Cells |
Synonyms | Cd28, CH29-189K17.3 |
Molecular Weight | The recombinant mouse CD28/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 378 amino acids and has a predicted molecular mass of 43 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD28/Fc monomer is approximately 55-60 kDa due to glycosylation. |
predicted N | Asn 20 |
SDS-PAGE | |
Purity | > 96 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Lys 149) of mouse CD28 (NP_031668.3) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. |
Bio-activity | Measured by its binding ability in a functional ELISA . 1. Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 Fc Chimera with a linear ranger of 6.4-800 ng/ml . 2. Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 Fc Chimera with a linear ranger of 6.4-160 ng/ml. |
Research Area | Immunology |Adaptive Immunity |T Cell |T Cell CD Antigen |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. |
Reference |