Mouse CD300A Protein (Fc Tag)
B230315M08Rik,Clm8,LMIR1,MAIR-I,MAIR-Ia,mcpir1,MMAC8,Pigr4
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| Catalog Number | P50675-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | B230315M08Rik,Clm8,LMIR1,MAIR-I,MAIR-Ia,mcpir1,MMAC8,Pigr4 |
| Molecular Weight | The recombinant mouse CD300A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 397 amino acids and has a predicted molecular mass of 44.2 kDa. The apparent molecular mass of rmCD300A/Fc monomer is approximately 55-65 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Leu 28 |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse CD300A (Q6SJQ0-1) (Met 1-Arg 183) extracellular domain was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | |
| Research Area | Signaling |Signal Transduction |ITIM/ITAM Immunoreceptors and Related Molecules |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Mouse CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains one Ig-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs). |
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