Call Now

Mouse CD34 Protein (His Tag)

AU040960

Catalog Number P50589-M08H
Organism Species Mouse
Host Human Cells
Synonyms AU040960
Molecular Weight The recombinant mouse CD34 consists of 264 amino acids and has a predicted molecular mass of 28.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCD34 is approximately 50-60 kDa due to glycosylation.
predicted N Thr 35
SDS-PAGE
Purity > 98 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain of mouse CD34 (NP_001104529.1) (Met 1-Thr 287) was expressed, with a polyhistidine tag at the C-terminus.
Bio-activity Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line . When 4 x 10E4 cells/well are added to mouse CD34 coated plates (0.8 μg/ml, 100 μl/well), approximately >30 % will adhere after one hour at 37 ℃.
Research Area Developmental Biology |Embryogenesis |Germ Layer Formation |Mesoderm
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
Reference
  • Hogan CJ, et al. (2002) Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice. Proc Nat Acad Sci USA. 99 (1): 413-8.
  • Nielsen JS,et al. (2009) CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery. Microcirculation. 16(6): 487-96.
  • Mastrandrea F,et al. (2009) CD34+ hemopoietic precursor and stem cells traffic in peripheral blood of celiac patients is significantly increased but not directly related to epithelial damage severity. Eur Ann Allergy Clin Immunol. 40(3): 90-103.
  • Pasquet S,et al. (2009) Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct. Cytotherapy. 11(8): 1002-15.