Mouse CD3D & CD3E Heterodimer Protein

T3d

Catalog Number	PCT025-M2303H
Organism Species	Mouse
Host	Human Cells
Synonyms	T3d
Molecular Weight	The recombinant heterodimer of mouse CD3D/CD3E comprises 670 (333+337) amino acids and has a calculated molecular mass of 76 (38+38） kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
predicted N	Phe 22 & Asp 22
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
Bio-activity
Research Area
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference