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Mouse CD40 / TNFRSF5 Protein (His & Fc Tag)

AI326936,Bp50,GP39,HIGM1,IGM,IMD3,p50,T-BAM,Tnfrsf5,TRAP

Catalog Number P50324-M03H
Organism Species Mouse
Host Human Cells
Synonyms AI326936,Bp50,GP39,HIGM1,IGM,IMD3,p50,T-BAM,Tnfrsf5,TRAP
Molecular Weight The secreted recombinant mouse CD40/Fc is a disulfide-linked homodimer The reduced monomer comprises 422 amino acids and has a predicted molecular mass of 47 kDa. As a result of glycosylation, it migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
predicted N Leu 20
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain of mouse CD40 isoform 1 (P27512-1) (Met 1-Arg 193) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Bio-activity Measured by its ability to bind recombinant human CD40L in a functional ELISA.
Research Area Immunology |Innate Immunity |Cytokine |TNF Superfamily |Processes Regulated by TNF Superfamily Members |Regulation of T Cell Co-stimulation by TNF Superfamily Members |
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Reference
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.