Mouse CD5 / LEU1 Protein (His Tag)

Ly-1,Ly-12,Ly-A,Lyt-1

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Catalog Number	P50403-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	Ly-1,Ly-12,Ly-A,Lyt-1
Molecular Weight	The secreted recombinant mouse CD5 consists of 359 amino acids and has a calculated molecular mass of 39.4 kDa. As a result of glycosylation, the apparent molecular mass of rmCD5 is approximately 42-48 kDa in SDS-PAGE under reducing conditions.
predicted N	Gln 24
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of mouse CD5 (NP_031676.3) (Met 1-Pro 371) was expressed, with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Immunology |Inflammation / Inflammatory Mediator |Cells Involved in Inflammation |Monocyte/Macrophage
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.
Reference	Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters. 134 (2): 104-12. Kirchgessner H, et al. (2001) The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 193 (11): 1269-84.