Mouse CD6 / TP120 Protein (Fc Tag)
CD6
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| Catalog Number | P50711-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | CD6 |
| Molecular Weight | The recombinant mouse CD6/Fc is a disulfide-linked homodimer. The reduced monomer comprises 621 amino acids and has a calculated molecular mass of 68.2 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Gly 17 |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of mouse CD6 (Q91WN5) (Met 1-Val 243) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 104 cells/well are added to mCD6-Fc coated plates (10μg/mL, 100 μL/well), approximately 14.4% will adhere after 60 minutes at 37℃. |
| Research Area | Immunology |Inflammation / Inflammatory Mediator |Cells Involved in Inflammation |Monocyte/Macrophage |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse. |
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