Mouse CD80 / B7-1 / CD28LG Protein (His Tag)
B71,Cd28l,Ly-53,Ly53,MIC17,TSA1
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| Catalog Number | P50446-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | B71,Cd28l,Ly-53,Ly53,MIC17,TSA1 |
| Molecular Weight | The recombinant mouse CD80 consists of 219 amino acids and has a predicted molecular mass of 25.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD80 is approximately 48-50 kDa due to glycosylation. |
| predicted N | Val 38 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was expressed, with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to bind recombinant mouse CD28 in a functional ELISA. |
| Research Area | Developmental Biology |Embryogenesis |Germ Layer Formation |Ectoderm Marker |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. |
| Reference |