Mouse CD80 / B7-1 Protein (Fc Tag)
B71,Cd28l,Ly-53,Ly53,MIC17,TSA1
- 100ug (NPP1181) Please inquiry
| Catalog Number | P50446-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | B71,Cd28l,Ly-53,Ly53,MIC17,TSA1 |
| Molecular Weight | The recombinant mouse CD80 consists of 449 amino acids and has a predicted molecular mass of 50.7 kDa. |
| predicted N | Val 38 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human CTLA4 (cat: 11159-H08H) at 10 μg/mL (100 μl/well) can bind mouse CD80-Fc (cat: 50446-M02H), The EC50 of mouse CD80-Fc (cat: 50446-M02H) is 28 ng/mL. |
| Research Area | Developmental Biology |Embryogenesis |Germ Layer Formation |Ectoderm Marker |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. |
| Reference |