Mouse CHK2 / CHEK2 Protein (His & GST Tag)

CHK2, Cds1, HUCDS1, Rad53

Catalog Number	P50432-M20B
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	CHK2, Cds1, HUCDS1, Rad53
Molecular Weight	The secreted recombinant mouse CHEK2/GST chimera consists of 783 amino acids and has a calculated molecular mass of 89 kDa. The recombinant protein migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse CHEK2 (Q9Z265) (Mey 1-Leu 546) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	Kinase activity untested
Research Area	Cancer \|Signal transduction \|Other Related Intracellular Topics \|Cellular Senescence and Pathways in Aging \|DNA Damage and Repair
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Bogdanova N, et al. (2005) Association of two mutations in the CHEK2 gene with breast cancer. Cancer Genetics. 116(2) : 263-6. Dong XY, et al. (2003) Mutations in CHEK2 associated with prostate cancer risk. The American journal of human genetics. 72(2) 270-80.