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Mouse CXADR / CAR Protein

2610206D03Rik,AU016810,AW553441,CAR,MCAR,MCVADR

Catalog Number P50019-MCCH
Organism Species Mouse
Host Human Cells
Synonyms 2610206D03Rik,AU016810,AW553441,CAR,MCAR,MCVADR
Molecular Weight The recombinant mouse CXADR consists of 225 amino acids and has a calculated molecular mass of 25 kDa. The recombinant protein migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
predicted N Leu 20
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mouse CXADR (NP_001020363.1) (Met 1-Gly 237) was expressed with six amino acids (ENLYFQ) at the C-terminus.
Bio-activity
Research Area Signaling |Signal Transduction |Cytoskeleton / ECM |Cell Adhesion |Tight Junctions
Formulation Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I  transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.
Reference
  • Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352–3356.
  • van Raaij , M.J. et al., 2001, Structure. 8 (11): 1147–1155.
  • Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392–25398.
  • Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219–226.
  • Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127–131.
  • Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111.
  • Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.