Mouse Carboxypeptidase B1 / CPB1 Protein (His Tag)
0910001A18Rik,1810063F02Rik,2210008M23Rik,AI504870
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| Catalog Number | P50386-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | 0910001A18Rik,1810063F02Rik,2210008M23Rik,AI504870 |
| Molecular Weight | The secreted recombinant mouse CPB1 comprises 411 amino acids with a predicted molecular mass of 47.5 kDa. The apparent molecular mass of rm CPB1 is approximately 43-45 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ser 16 |
| SDS-PAGE |  |
| Purity | > 94 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse CPB1 (NP_083982.1) precursor (Met 1-Tyr 415) was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg . The specific activity is >5,000 pmoles/min/μg. |
| Research Area | Immunology |Innate Immunity |Coagulation |Regulatory |
| Formulation | Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin. |
| Reference |