Mouse ECM1 Protein (His Tag)

AI663821,p85

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Catalog Number	P50331-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	AI663821,p85
Molecular Weight	The secreted recombinant mouse ECM1 comprises 551 amino acids and has a predicted molecular mass of 62.5 kDa. As a result of glycosylation, the apparent molecular mass of rm ECM1 is approximately 90-95 kDa in SDS-PAGE under reducing conditions.
predicted N	Ala 20
SDS-PAGE
Purity	> 88 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse ECM1 isoform 1 (Q61508-1) (Met 1-Glu 559) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to bind human MMP-9 in a functional ELISA.
Research Area	Cardiovascular |Lipids / Lipoproteins |Lipid Metabolism |Cytochromes
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.
Reference	Oyama N, et al. (2003) Autoantibodies to extracellular matrix protein 1 in lichen sclerosus. Lancet. 362(9378): 118-23. Chan I, et al. (2004) Rapid diagnosis of lipoid proteinosis using an anti-extracellular matrix protein 1 (ECM1) antibody. J Dermatol Sci. 35(2): 151-3. Lupo I, et al. (2005) A novel mutation of the extracellular matrix protein 1 gene (ECM1) in a patient with lipoid proteinosis (Urbach-Wiethe disease) from Sicily. Br J Dermatol. 153(5): 1019-22. Sander CS, et al. (2006) Expression of extracellular matrix protein 1 (ECM1) in human skin is decreased by age and increased upon ultraviolet exposure. Br J Dermatol. 154(2): 218-24.