Mouse EGFR / HER1 / ErbB1 Protein (Fc Tag)
9030024J15Rik,AI552599,Erbb,Errb1,Errp,wa-2,wa2,Wa5
- 100ug (NPP1242) Please inquiry
| Catalog Number | P51091-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | 9030024J15Rik,AI552599,Erbb,Errb1,Errp,wa-2,wa2,Wa5 |
| Molecular Weight | The secreted recombinant mouse EGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 864 amino acids and has a calculated molecular mass of 96 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR/Fc monomer is approximately 130 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Leu 25 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its ability to bind human EGF (P10605-HNAE) in a functional ELISA. |
| Research Area | Signaling |Signal Transduction |Protein Phosphorylation |Tyrosine Kinase |Receptor Tyrosine Kinases |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. |
| Reference |