Mouse ENPEP / Aminopeptidase A Protein (His Tag)

6030431M22Rik,APA,Bp-1/6C3,ENPEP,Ly-51,Ly51

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Catalog Number	P50082-M07B
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	6030431M22Rik,APA,Bp-1/6C3,ENPEP,Ly-51,Ly51
Molecular Weight	The recombinant mouse ENPEP consists of 921 amino acids and predicts a molecular mass of 106 KDa. It migrates as an approximately 113 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 91 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of mouse ENPEP (P16406) (Arg41-Pro945) was expressed, with a polyhistidine tag at the N-terminus.
Bio-activity	Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin. The specific activity is > 200 pmoles/min/μg.
Research Area	Signaling |Signal Transduction |Hormones
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.
Reference	Speth RC, et al. (2008) The significance of brain aminopeptidases in the regulation of the actions of angiotensin peptides in the brain. Heart Fail Rev. 13(3):299-309. Rose JE, et al. (2010) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Mol Med. 16(7-8):247-53. Pérez I, et al. (2009) Increased APN/CD13 and acid aminopeptidase activities in head and neck squamous cell carcinoma. Head Neck. 31(10):1335-40.