Mouse ESAM / Endothelial Cell Adhesion Molecule Protein (His Tag)

2310008D05Rik,Esam1,W117m

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Catalog Number	P50977-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	2310008D05Rik,Esam1,W117m
Molecular Weight	The secreted recombinant mouse ESAM comprises 233 amino acids and has a calculated molecular mass of 25.6 kDa. As a result of different glycosylation, the apparent molecular mass of the recombinant protein is approximately 33-43 kDa in SDS-PAGE under reducing conditions.
predicted N	Gln 30
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse ESAM (Q925F2) (Met 1-Ala 251) was expressed, with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Cancer |Invasion microenvironment |Adhesion molecule |Cell adhesion |Cell Adhesion Molecules |Endothelial |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.
Reference	Ishida T, et al. (2003) Targeted disruption of endothelial cell-selective adhesion molecule inhibits angiogenic processes in vitro and in vivo. J Biol Chem. 278(36): 34598-604. Wegmann F, et al. (2004) Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp Cell Res. 300(1): 121-33. Wegmann F, et al. (2006) ESAM supports neutrophil extravasation, activation of Rho, and VEGF-induced vascular permeability. J Exp Med. 203(7): 1671-7. Hara T, et al. (2009) Endothelial cell-selective adhesion molecule regulates albuminuria in diabetic nephropathy. Microvasc Res. 77(3): 348-55. Cangara HM, et al. (2010) Role of endothelial cell-selective adhesion molecule in hematogeneous metastasis. Microvasc Res. 80(1): 133-41.