Mouse EphA7 / EHK-3 Protein (His Tag)
Cek11,Ebk,Ehk3,Hek11,Mdk1,RP23-33D17.1
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| Catalog Number | P50587-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | Cek11,Ebk,Ehk3,Hek11,Mdk1,RP23-33D17.1 |
| Molecular Weight | The secreted recombinant mouse EPHA7 consists of 537 amino acids and has a predicted molecular mass of 60.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA7 is approximately 70 kDa due to glycosylation. |
| predicted N | Lys 30 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse EPHA7 isoform 1 (Q61772-1) extracellular domain (Met 1-Ile 556) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized mouse EphA7 at 2 μg/ml (100 μl/well) can bind mouse EphrinA4 with a linear range of 0.08-10 ng/ml . |
| Research Area | Cancer |Signal transduction |Growth Factor & Receptor |Ephrin & Eph Receptor |Eph Receptor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas. |
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