Mouse EphB4 / HTK Protein (Fc Tag)

AI042935,Htk,MDK2,Myk1,Tyro11

100ug (NPP2719) Please inquiry

Catalog Number	P50582-M02H
Organism Species	Mouse
Host	Human Cells
Synonyms	AI042935,Htk,MDK2,Myk1,Tyro11
Molecular Weight	The secreted recombinant mouse EPHB4/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 765 amino acids and has a predicted molecular mass of 84.7 kDa. As a result of glycosylation, the apparent molecular mass of rm EPHB4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions.
predicted N	Leu 16
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHB2-His (P50598-M08H) at 10 μg/mL (100 μl/well) can bind mouse EPHB4-Fc (P50582-M02H), The EC₅₀ of mouse EPHB4-Fc (P50582-M02H) is 15 ng/mL.
Research Area	Epigenetics \|Transcription \|Cancer susceptibility \|Tumor Suppressor
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
Reference	Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8. Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21. Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8. Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7. Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.