Mouse EphB4 / HTK Protein (His Tag)
AI042935,Htk,MDK2,Myk1,Tyro11
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| Catalog Number | P50582-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | AI042935,Htk,MDK2,Myk1,Tyro11 |
| Molecular Weight | The secreted recombinant mouse EPHB4 comprises 535 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, the apparent molecular mass of rm EPHB4/Fc monomer is approximately 70 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Leu 16 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized mouse EphB4 at 2 μg/ml (100 μl/well) can bind mouse EphrinB2 with a linear range of 0.2-25 ng/ml. |
| Research Area | Epigenetics |Transcription |Cancer susceptibility |Tumor Suppressor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
| Reference |