Mouse FGFR1 / CD331 Protein (His Tag)

AW208770,bFGF-R-1,c-fgr,Eask,Fgfr-1,FLG,Flt-2,Hspy,MFR

100ug (NPP3314) Please inquiry

Catalog Number	P50186-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	AW208770,bFGF-R-1,c-fgr,Eask,Fgfr-1,FLG,Flt-2,Hspy,MFR
Molecular Weight	The secreted recombinant mouse FGFR1 consists of 366 amino acids and has a calculated molecular mass of 40.9 kDa. The apparent molecular mass of rmFGFR1 is approximately 55-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Arg 22
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Glu 376) of mouse FGFR1 isoform 1 (P16092-1) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 50-200 ng/ml.
Research Area	Cancer \|Signal transduction \|Growth Factor & Receptor \|Fibroblast Growth Factor (FGF) & Receptor \|FGF Receptor
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ，idiopathic hypogonadotropic hypogonadism， Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.
Reference	Schlessinger J, et al. (2000) Crystal structure of a ternary FGF-FGFR-heparin complex reveals a dual role for heparin in FGFR binding and dimerization. Mol Cell. 6(3):743-50. Dodé C, et al. (2007) Novel FGFR1 sequence variants in Kallmann syndrome, and genetic evidence that the FGFR1c isoform is required in olfactory bulb and palate morphogenesis. Hum Mutat. 28(1): 97-8. Kim HG, et al. (2005) Hypogonadotropic hypogonadism and cleft lip and palate caused by a balanced translocation producing haploinsufficiency for FGFR1. J Med Genet. 42(8):666-72.