Mouse FLT-3 / CD135 / FLK-2 Protein (His Tag)

B230315G04,CD135,Flk-2,Flk2,Flt-3,Ly72,wmfl

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Catalog Number	P51119-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	B230315G04,CD135,Flk-2,Flk2,Flt-3,Ly72,wmfl
Molecular Weight	The recombinant mouse FLT3 comprises 528 amino acids and has a predicted molecular mass of 59.5 kDa.
predicted N	Asn 28
SDS-PAGE
Purity	> 75 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse FLT3 (EDL05833.1) (Met1-Ser544) was expressed with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Signaling |Signal Transduction |Receptor Tyrosine Kinases (RTKs)
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeⅢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.
Reference	Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. Bertho, et al. (2000) CD135 (Flk2 / Flt3) Expression by Human Thymocytes Delineates a Possible Role of FLT3-Ligand in T-Cell Precursor Proliferation and Differentiation. Scandinavian Journal of Immunology. 52 (1): 53-61.