Mouse Frizzled-5 / FZD5 Protein (Fc Tag)
5330434N09Rik,AI427138,Fz-5,Fz5,mFz5
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| Catalog Number | P50772-M02S |
|---|---|
| Organism Species | Mouse |
| Host | CHO Stable Cells |
| Synonyms | 5330434N09Rik,AI427138,Fz-5,Fz5,mFz5 |
| Molecular Weight | The recombinant mouse FZD5 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 382 amino acids and has a predicted molecular mass of 42.9 kDa. The apparent molecular mass of the protein is approximately 52-56 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Ala 27 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse FZD5 (Q9EQD0) (Met1-Pro167) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | |
| Research Area | Signaling |Signal Transduction |G Protein-Coupled Receptors (GPCRs) |Frizzled Receptors |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 10 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt10B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus. |
| Reference |