Mouse GFRA3 / GFR-alpha-3 Protein (Fc Tag)
GFRalpha3,Y15110
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| Catalog Number | P50901-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | GFRalpha3,Y15110 |
| Molecular Weight | The secreted recombinant mouse GFRA3 Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and has a calculated molecular mass of 66.2 kDa. The apparent molecular mass of rmGFRA3/Fc monomer is approximately 70 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Asn 29 |
| SDS-PAGE |  |
| Purity | > 80 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse GFRA3 (O35118) (Met 1-Arg 379), was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | |
| Research Area | Cancer |Invasion microenvironment |Angiogenesis |Cytokine & Receptor |Transforming Growth Factor Beta (TGF-beta) Superfamily |TGF-beta Superfamily Receptors | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor α3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons. |
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