Mouse ICAM-2 / CD102 Protein (Fc Tag)
CD102,Icam-2,Ly-60
- 100ug (NPP3356) Please inquiry
| Catalog Number | P50644-M02H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | CD102,Icam-2,Ly-60 |
| Molecular Weight | The secreted recombinant mouse ICAM2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 444 amino acids and has a predicted molecular mass of 50 kDa. As a result of glycosylation, the apparent molecular mass of rm ICAM2/Fc monomer is approximately 60-70 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ser 20 |
| SDS-PAGE |  |
| Purity | > 93 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse ICAM2 (NP_034624.1) extracellular domain (Met 1-Gln 222) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells . When cells are added to mouse ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 25-40% will adhere specifically. |
| Research Area | Cardiovascular |Vasculature |Endothelium |Endothelial Cell Markers |
| Formulation | Lyophilized from sterile 50mM Tris-Citric acid, 150mM NaCl, pH 5.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment. |
| Reference |