Mouse IL-23 (IL23A & IL12B Heterodimer) Protein
IL-23,p19
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| Catalog Number | PCT028-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | IL-23,p19 |
| Molecular Weight | The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions. |
| predicted N | Val 22 & Met 23 |
| SDS-PAGE |  |
| Purity | (33.1+31.5+32.9) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified. |
| Bio-activity | Measured by its binding ability in a functional ELISA. 1. Measured by its ability to bind recombinant rat IL23R-Fc (P80395-R02H) in a functional ELISA. 2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind human IL23R-Fc (P13840-H02H). The EC50 of human IL23R-Fc (P13840-H02H) is 0.14-0.35 μg/ml. 3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc (P90123-C02H). The EC50 of Cynomolgus IL23R-Fc (P90123-C02H) is 0.05-0.11 μg/ml. 4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (P11674-H08H). The EC50 of biotinylated human IL12RB1-His (P11674-H08H) is 21.3-49.8 ng/mL. 5. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 1-5 ng/mL. |
| Research Area | Cancer |Invasion microenvironment |Adhesion molecule |Extracelluar matrix |others in ECM |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
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