Mouse IL12RB2 / IL12R-beta 2 Protein (His Tag)
A930027I18Rik,Ifnm,IL-12RB2
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| Catalog Number | P50099-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | A930027I18Rik,Ifnm,IL-12RB2 |
| Molecular Weight | The secreted recombinant mouse IL12RB2 consists of 625 amino acids and has a predicted molecular mass of 70 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIL12RB2 is approximately 120-130 kDa due to glycosylation. |
| predicted N | Asn 24 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse IL12RB2 (NP_032380.1) extracellular domain (Met 1-Asn 637) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to bind Mouse IL12A & IL12B Heterodimer Protein (PCT023-M0802H) in a functional ELISA. Immobilized mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A & IL12B Heterodimer Protein (PCT024-M0208H). The EC50 of Mouse IL12A & IL12B Heterodimer Protein (PCT024-M0208H) is 50.2-117.2 ng/ml. |
| Research Area | Cardiovascular |Angiogenesis |Cytokines / Chemokines in Angiogenesis |Interleukins |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin-12 receptor subunit beta-2 (IL12RB2), also known as IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, and IL-12RB2, is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. IL12RB2 belongs to the type I cytokine receptor family. The coexpression of IL12RB2 and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of IL12RB2 is up-regulated by IFN gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of IL12RB2 is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. IL12RB2 promotes the proliferation of T-cells as well as NK cells. IL12RB2 induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. |
| Reference |