Mouse IL13RA2 / CD213A2 Protein (His Tag)
CD213a2
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| Catalog Number | P50061-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | CD213a2 |
| Molecular Weight | The recombinant mouse IL13Rα2 consists of 324 amino acids after removal of the signal peptide and has a predicted molecular mass of 38 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIL13Rα2 is approximately 45-50 kDa due to glycosylation. |
| predicted N | Leu 22 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Lys 334) of mouse IL13Rα2 (NP_032382.1) precursor was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140: 323. The ED50 for this effect is typically 0.1-0.5 μg/mL. |
| Research Area | Cancer |Invasion microenvironment |Angiogenesis |Cytokine & Receptor |Interleukin & Receptor |Other Interleukin & Receptor | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors. |
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