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Mouse IL7 / Interleukin 7 Protein

A630026I06Rik,hlb368,Il-7

Catalog Number P50217-MNAE
Organism Species Mouse
Host E. coli
Synonyms A630026I06Rik,hlb368,Il-7
Molecular Weight The recombinant mouse IL7 consists of 130 amino acids and predicts a molecular mass of 15 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mouse IL7 (NP_032397.1) (Glu26-Ile154) was expressed and purified with an initial Met.
Bio-activity Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL).
The ED50 for this effect is typically 1.3-5.2 ng/mL.
Research Area Immunology |Inflammation / Inflammatory Mediator |Inflammatory Cytokines & Chemoki and Receptors |Common gamma Chain Receptor Family
Formulation Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background IL7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.
Reference
  • Watanabe M, et al. (1995) Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of intestinal mucosal lymphocytes.
  • J Clin Invest. 95(6):2945-53. Sawa Y, et al. (2009) Hepatic interleukin-7 expression regulates T cell responses. Immunity. 30 (3):447-57.
  • Flad HD, et al. (1996) Human follicular dendritic cells and vascular cells produce interleukin-7: a potential role for interleukin-7 in the germinal center reaction. Eur J Immunol. 26(10): 2541-4.