Mouse KIRREL1 / NEPH1 Protein (His Tag)
6720469N11Rik,Kirrel1,Neph1
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| Catalog Number | P50193-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | 6720469N11Rik,Kirrel1,Neph1 |
| Molecular Weight | The secreted recombinant mouse KIRREL1 consists of 489 amino acids and has a calculated molecular mass of 53.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions. |
| predicted N | Leu 48 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of mouse KIRREL1 (NP_570937.2) (Met 1-Leu 525) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells (ATCC: CRL2279). When cells are added to coated plates (30 μg/mL, 100 μL/well), > 40% cells will adhere specifically after 90 minutes at 37 ℃. |
| Research Area | Immunology |Signal Transduction |Protein Phosphorylation |Tyrosine Kinase |Tec Family |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death. |
| Reference |