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Mouse KLK1 / Kallikrein 1 Protein (His Tag)

0610007D04Rik,Kal,Klk1b6,Klk6,mGk-6

Catalog Number P50915-M08H
Organism Species Mouse
Host Human Cells
Synonyms 0610007D04Rik,Kal,Klk1b6,Klk6,mGk-6
Molecular Weight The recombinant mouse KLK1 comprises 254 amino acids and has a predicted molecular mass of 28.3 kDa. The apparent molecular mass of the protein is approximately 36 kDa in SDS-PAGE under reducing conditions.
predicted N Pro 19
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mouse KLK1 (P15947) (Met1-Asp261) was expressed with a C-terminal polyhistidine tag.
Bio-activity Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin(PFR-AMC).
The specific activity is >6, 000 pmol/min/μg.
(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Research Area Immunology |Inflammation / Inflammatory Mediator |Plasma Cascade Systems in Inflammation |Fibrinolysis System
Formulation Lyophilized from sterile 25 mM Tris, 5 mM CaCl2, 0.15 M NaCl, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference