Mouse LAIR1 Protein (Fc Tag)

5133400O11Rik,BB115266,D7Bwg0421e,Lair-1

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Catalog Number	P51030-M02H
Organism Species	Mouse
Host	Human Cells
Synonyms	5133400O11Rik,BB115266,D7Bwg0421e,Lair-1
Molecular Weight	The recombinant mouse LAIR1 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 353 amino acids and has a predicted molecular mass of 39.6 kDa. The apparent molecular mass of the protein is approximately 49-53 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Gln 22
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse LAIR1 (Q8BG84-6) (Gln22-Ser133) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity
Research Area	Immunology |Cluster of Differentiation (CD) |T Cell CD Antigen |CD Antigen (T Cell Migration/Adhesion)
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Leukocyte associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and are able to spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells, but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells.
Reference	Meyaard L, et al. (1999) Leukocyte-associated Ig-like receptor-1 functions as an inhibitory receptor on cytotoxic T cells. J Immunol. 162 (10): 5800-4. Meyaard L, et al. (2001) The epithelial cellular adhesion molecule (Ep-CAM) is a ligand for the leukocyte-associated immunoglobulin-like receptor (LAIR). J Exp Med. 194 (1): 107-12. Xu M, et al. (2000) Identification and characterization of leukocyte-associated Ig-like receptor-1 as a major anchor protein of tyrosine phosphatase SHP-1 in hematopoietic cells. J Biol Chem. 275 (23): 17440-6.