Mouse NBL1 / DAND1 / DAN Protein (His Tag)
D4H1S1733E,DAN,Dana,NO3
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| Catalog Number | P50976-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | D4H1S1733E,DAN,Dana,NO3 |
| Molecular Weight | The secreted recombinant mouse NBL1 comprises 173 amino acids and has a calculated molecular mass of 18.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ala 17 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse NBL1 (Q61477) (Met 1-Asp 178) was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | |
| Research Area | Cancer |Oncoprotein & suppressor & biomarker |Tumor biomarker |Tumor Suppressor |Other in Tumor suppressor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma. |
| Reference |