Mouse Notch-1 / NOTCH1 Protein (His Tag)
9930111A19Rik,lin-12,Mis6,N1,Tan1
- 100ug (NPP2766) Please inquiry
| Catalog Number | P50654-M08B |
|---|---|
| Organism Species | Mouse |
| Host | Baculovirus-Insect Cells |
| Synonyms | 9930111A19Rik,lin-12,Mis6,N1,Tan1 |
| Molecular Weight | The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | Ala 19 |
| SDS-PAGE |  |
| Purity | > 82 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his (P50640-M08H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL. |
| Research Area | Signaling |Signal Transduction |Signaling Pathway |Representative pathway |Notch Signaling pathway |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |