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Mouse PGK1 / PGKA Protein (His Tag)

MGC118097, Pgk-1

Catalog Number P50628-M08B
Organism Species Mouse
Host Baculovirus-Insect Cells
Synonyms MGC118097, Pgk-1
Molecular Weight The recombinant mouse PGK1 consists of 427 amino acids and has a calculated molecular mass of 46 kDa. It migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mouse PGK1 (P09411) (Met 1-Val 417) was expressed, with a C-terminal polyhistidine tag.
Bio-activity Kinase activity untested
Research Area Immunology |Signal Transduction |Metabolism |Pathways and Processes |Metabolic signaling pathways |Carbohydrate metabolism |
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference
  • Rush J. et al., 2005, Nat Biotechnol. 23: 94-101.
  • Olsen JV. et al., 2006, Cell. 127: 635-648.
  • Zieker D. et al., 2008, Cell Physiol Biochem. 21 (5-6): 429-36.
  • Jung Y. et al., 2009, Mol Cancer Res. 7 (10): 1595-604.
  • Choudhary C. et al., 2009, Science. 325: 834-40.