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Mouse PLA2G2E Protein (His Tag)

RP23-352K10.7

Catalog Number P50687-M08H
Organism Species Mouse
Host Human Cells
Synonyms RP23-352K10.7
Molecular Weight The secreted recombinant mouse PLA2G2E comprises 134 amino acids and has a calculated molecular mass of 15.5 kDa. The apparent molecular mass of the recombinant protein is approximately 18 kDa in SDS-PAGE under reducing conditions.
predicted N Asn 20
SDS-PAGE
Purity > 97 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mouse PLA2G2E (Q9QUL3) (Met 1-Cys 142) was expressed, with a C-terminal polyhistidine tag.
Bio-activity
Research Area Signaling |Signal Transduction |Metabolism |Types of disease |Metabolism in Cancer
Formulation Lyophilized from sterile PBS, 10% glycerol, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Mouse Group IIE secretory phospholipase A2, also known as GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine 2-acylhydrolase 2E and PLA2G2E is a secreted protein which belongs to the phospholipase A2 family. Mammalian secretory phospholipase A2s ( sPLA2s ) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2 / PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2 / PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2 / PLA2. Due to the increased presence and activity of Phospholipases A2 / PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site. PLA2G2E catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
Reference
  • Dennis EA. et al.,1994, J. Biol. Chem. 269 (18): 13057-60. 
  • Nicolas JP, et al.,1997, J. Biol. Chem. 272 (11): 7173-81.
  • Six DA, et al., 2000, Biochim. Biophys. Acta 1488 (1-2): 1-19.
  • Suzuki N., et al., 2000,J. Biol. Chem. 275:5785-93.