Mouse Pleiotrophin / PTN / HB-GAM Protein

HARP,HB-GAM,HBBN,HBGF-8,HBNF,OSF,Osf-1,Osf1

• 100ug (NPP3449) Please inquiry

Catalog Number	P51000-MNAB
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	HARP,HB-GAM,HBBN,HBGF-8,HBNF,OSF,Osf-1,Osf1
Molecular Weight	The recombinant mouse PTN consists of 136 amino acids and predicts a molecular mass of 15.3 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
predicted N	Gly 33
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse PTN (P63089) (Met1-Asp168) was expressed.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc (P80344-R02H), The EC50 of rat SDC1-Fc (P80344-R02H) is 0.4-1.1 μg/ml.
Research Area	Immunology |Signal Transduction |Hormones
Formulation	Lyophilized from sterile 20 mM Tris, 1M NaCl, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.
Reference	Vanderwinden JM, et al. (1992) Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues. Anat Embryol. 186(4):387-406. Lauri SE, et al. (1996) Activity-induced enhancement of HB-GAM expression in rat hippocampal slices. Neuroreport. 7(10):1670-4. Pavlov I, et al. (2002) Role of heparin-binding growth-associated molecule (HB-GAM) in hippocampal LTP and spatial learning revealed by studies on overexpressing and knockout mice. Mol Cell Neurosci. 20(2):330-42.