Mouse SHH / Sonic hedgehog Protein (His Tag)
9530036O11Rik,Dsh,Hhg1,Hx,Hxl3,M100081
- 100ug (NPP3487) Please inquiry
| Catalog Number | P51100-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | 9530036O11Rik,Dsh,Hhg1,Hx,Hxl3,M100081 |
| Molecular Weight | The recombinant mouse SHH comprises 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Cys 25 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse SHH (Q62226) (Met1-Gly198) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 5-40 μg/mL. |
| Research Area | Immunology |Signal Transduction |Signaling Pathway |Representative pathway |Hedgehog Signaling Pathway |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3. |
| Reference |