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Mouse Semaphorin 4D / SEMA4D / CD100 Protein (His Tag)

CD100,coll-4,Semacl2,Semaj,Semcl2

Catalog Number P50642-M08H
Organism Species Mouse
Host Human Cells
Synonyms CD100,coll-4,Semacl2,Semaj,Semcl2
Molecular Weight The secreted recombinant mouse SEMA4D comprises 721 amino acids and has a calculated molecular mass of 80.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
predicted N Phe 24
SDS-PAGE
Purity > 96 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the mouse SEMA4D (NP_038688.2) extracellular domain (Met 1-Arg 733) was expressed, with a C-terminal polyhistidine tag.
Bio-activity Measured by its ability to bind human SEMA4A in a functional ELISA.
Research Area Neuroscience |Neurology process |Growth and Development |Axon Guidance |Semaphorin, Plexin, & Neuropilin
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference