Mouse SerpinF1 / PEDF Protein (His Tag)
AI195227,EPC-1,Pedf,Pedfl,Sdf3
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| Catalog Number | P50235-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | AI195227,EPC-1,Pedf,Pedfl,Sdf3 |
| Molecular Weight | The secreted recombinant mouse SerpinF1consists of 409 amino acids and has a calculated molecular mass of 45.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions. |
| predicted N | Gln 20 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Thr 417) of mouse SerpinF1 (NP_035470.3) precursor was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized mouse SERPINF1-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CSNK2A1 (P10630-H09B) with a linear range of 0.31-2.5 μg/ml. |
| Research Area | Developmental Biology |Organogenesis |Skeletal development |Other Bone Remodeling Molecules |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression. |
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