Mouse TCN2 / Transcobalamin-II Protein (His Tag)
AW208754,Tcn-2
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| Catalog Number | P50693-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | AW208754,Tcn-2 |
| Molecular Weight | The secreted recombinant mouse TCN2 comprises 423 amino acids and has a calculated molecular mass of 47 kDa. The apparent molecular mass of the recombinant protein is approximately 42 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Glu 19 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mouse TCN2 (O88968) (Met 1-Trp 430) was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | |
| Research Area | Cancer |Signal transduction |Metabolism |Pathways and Processes |Vitamins / minerals |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities. |
| Reference |