Call Now

Mouse TROP2 / TACSTD2 Protein (Fc Tag)

C80403,EGP-1,GA733-1,Ly97,TROP2

Catalog Number P50922-M02H
Organism Species Mouse
Host Human Cells
Synonyms C80403,EGP-1,GA733-1,Ly97,TROP2
Molecular Weight The secreted recombinant mouse TACSTD2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 487 amino acids and has a calculated molecular mass of 55 kDa. As a result of glycosylation, the apparent molecular mass of rmTACSTD2/Fc monomer is approximately 66 kDa in SDS-PAGE under reducing conditions.
predicted N Gln 25
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain of mouse TACSTD2 (Q8BGV3?) (Met 1-Gln 270) was fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 10E4 cells/well are added to coated plates (40 μg/mL, 100 μL/well), > 50% cells will adhere after 1 hour at 37℃.
Research Area Cell Biology |Cell Cycle |Cell division |Other cell division related protein
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.
Reference
  • Ripani E, et al. (1998) Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 76(5): 671-6.
  • Wang J, et al. (2008) Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Mol Cancer Ther. 7(2): 280-5.